8/19/2023 0 Comments Bbc binaural soundSince the audio processing is happening in the browser, we can allow users to select their preferred headphone option without needing to provide multiple different streams. This is the way that surround sound sources are conventionally processed for listening on headphones (or a stereo loudspeaker system). We are also offering a stereo down-mix, where the surround and front-centre channels are added to the front-left and front-right signals to create a two-channel stereo version, as shown in the image below. In this experiment we have made two different binaural processing options available, with impulse responses measured in different acoustic environments. The Web Audio node graph for creating the binaural headphone surround For more background on binaural techniques, you can read this previous R&D blog post or this BBC News article. The image below shows the signal flow within the Web Audio API to create this effect, it was created using Firefox Web Audio Editor. T hese measurements are used to process each channel of the surround sound signal and the results are mixed together to form a two-channel signal for headphones. The resulting effect should be that of the original surround sound mix coming from five virtual loudspeakers, outside of the listener's head. We have measured impulse responses from a surround sound loudspeaker system using a dummy head microphone, these are two-channel measurements describing the transfer function from the sound source to each ear. Using binaural impulse responses, measured using a dummy head microphone, the convolution process can be used to create the impression that a sound source is coming from a specific point in space, when listening on headphones. The Web Audio API specification provides a helpful informative section on convolution. This allows a developer to convolve an audio signal with a chosen impulse response. The most important feature of the API for headphone surround is the ConvolverNode interface. We can then process this sound source through a Web Audio node graph. To create the headphone surround sound experience, we use the MediaElementAudioSourceNode to grab the multichannel audio signal coming from the HTML5 audio element. BBC R&D's Matthew Paradis is co-chairing with the W3C 's Audio Working Group, which is working on the specification. It has a range of applications, such as music creation and games, or even recreating the sounds of the Radiophonic Workshop. The new headphone surround sound feature is possible thanks to a web technology called the Web Audio API. This client-side script API introduces capabilities for audio signal processing in web applications. After the broadcast, the play will be available on-demand for 30 days. This Saturday we are again streaming the play online in 5-channel surround, this time using our MPEG-DASH player, which works directly in a page on the Radio 4 website. This production was mixed in surround sound and at the time a 5.1 stream was made available to our audience. This week's Saturday Drama on Radio 4 will be the highly-acclaimed 2003 production of Dylan Thomas' Under Milk Wood, in celebration of the centenary of the Welsh writer's birth.
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8/19/2023 0 Comments Youngest age of consentThe first recorded age-of-consent law dates from 1275 in England as part of its provisions on rape, the Statute of Westminster 1275 made it a misdemeanor to "ravish" a "maiden within age," whether with or without her consent. Judges sometimes honored marriages based on mutual consent at ages younger than seven: in contrast to established canon, there are recorded marriages of two- and three-year-olds. Marriage would then be valid as long as neither of the two parties annulled the marital agreement before reaching puberty, or if they had already consummated the marriage. There were authorities that said that such consent for entering marriage could take place earlier. In the 12th century, Gratian, the influential compiler of canon law in medieval Europe, accepted the age of puberty for marriage to be around twelve for girls and around fourteen for boys but acknowledged consent to be meaningful if both children were older than seven years of age. Modern historians have sometimes shown reluctance to accept evidence of young ages of marriage, dismissing it as a 'misreading' by a later copier of the records. Not only were the records relatively rare, but not all bothered to record the participants' ages, and it seems that the more complete the records are, the more likely they are to reveal young marriages. In England, for example, the only reliable data in the early modern period comes from property records made after death. Reliable data for ages at marriage is scarce. In most cases, this coincided with signs of puberty, menstruation for a woman, and pubic hair for a man. In traditional societies, the age of consent for a sexual union was a matter for the family to decide, or a tribal custom. History and social attitudes Traditional attitudes These factors all make age of consent an often confusing subject and a topic of highly charged debates. There are many "grey areas" in this area of law, some regarding unspecific and untried legislation, others brought about by debates regarding changing societal attitudes, and others due to conflicts between federal and state laws. Charges and penalties resulting from a breach of these laws may range from a misdemeanor, such as corruption of a minor, to what is popularly called statutory rape. The laws may also vary by the type of sexual act, the gender of the participants or other considerations, such as involving a position of trust some jurisdictions may also make allowances for minors engaged in sexual acts with each other, rather than a single age. It should not be confused with other laws regarding age minimums including, but not limited to, the age of majority, age of criminal responsibility, voting age, drinking age, and driving age.Īge of consent laws vary widely from jurisdiction to jurisdiction, though most jurisdictions set the age of consent in the range 14 to 18 (with the exceptions of Argentina and Niger which set the age of consent for 13, and 14 Muslim states that set the consent by marriage only). It has sometimes been used with other meanings, such as the age at which a person becomes competent to consent to marriage, but consent to sexual activity is the meaning now generally understood. : 1–2 Generally, a law will establish the age below which it is illegal to engage in sexual activity with that person. The term age of consent typically does not appear in legal statutes. The person below the minimum age is considered the victim, and their sex partner the offender, although some jurisdictions provide exceptions through " Romeo and Juliet laws" if one or both participants are underage, and are close in age. Consequently, an adult who engages in sexual activity with a person younger than the age of consent is unable to legally claim that the sexual activity was consensual, and such sexual activity may be considered child sexual abuse or statutory rape. The age of consent is the age at which a person is considered to be legally competent to consent to sexual acts. Sex offender registries in the United States.Sexual and reproductive health and rights.Sex workers' rights ( Decriminalization.Not to be confused with Marriageable age. 'Maggie Moore(s)' Streaming Movie Review: Stream It or Skip It? 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Though - if we know anything about Murphy - they’re going to connect 110 percent together like that… “But the only indication we received at the current ~thema~ of the season was the one story of fear “by the sea” the other is set by the beach.” Now, this is not the very first time we got for the new season because he shared a picture of this gorgeous beach with the title: “watch american horror story season 10 free. Well, last week, the man, the myth, the creator - Ryan Murphy - went to Instagram to reveal the title and subject of this upcoming show… and to utterly confound everyone… The video begins simple enough, showing the 10th season’s championship: Double Feature instantly. Whether you adore the show or are a big frightening cat, we everyone can acknowledge that the US Horror Story trilogy has some of the funniest marketing ever. While a lot of season 10 is still a secret, at least it’s fair to assume that when AHS: Double featured eventually reaches FX, we will travel towards the beach. Fans should thus not have their illusions that they will see any actors from past seasons yet. That sounds strange, and we’re interested officially! In the eighth season of Revelation fans like Asylum and Coven are gathered together, it is essential to observe that Murphy only stated that the performers will be returning - not the concepts. It is where my live is when I concentrate on it. On a sheet of paper I have a list of finaliters and I work on them every. “This is usually a secret process of how I develop the concept. I think it’s always America at the core of everything,” he stated. “”We dabbled with extraterrestrial, we played with space, we flirted with some more intriguing things than others. Some individuals I haven’t yet touched because I felt like, ‘Do I play for them?’ So far, I reached everyone to say ‘yes,’ so that was fantastic.” When it came to naming the particular subject that will bring these performers together, Murphy retained mother. “ “So, I reached out to several people discretely. We’ve been working on a concept for american horror storty season 10 full episodes that fans would want to come back because it’s about reuniting fan favored actors - since it could be our last season,” said Murphy in January to Deadline. When the entire cast was finally unveiled, a film showing a dreary beach was shown on the backdrop with Erwin Peck’s “Dark of Night. Now that we realize the season is going to travel from sea to sands, we think two distinct species like sirens and vampire will be shown.” Back before it had come out, the only that the Pose manufacturer stated about the next season was that it was a spectacular cast homecoming. 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Through this process, I connected with a whole new group of people that I did not know so well initially, and that was the most rewarding part of this whole experience.”Īaron Nichols is an art director, animator, editor and multidisciplinary designer based in Queens NY. I knew I had access to this world through Adam and the bands and I felt an overwhelming desire to direct this film. I wanted to instead capture the moment fresh as it was happening. Other documentaries I have worked on primarily were comprised of interviews of people talking about the old days. I knew this era of my life was coming to a close as the inevitable split of the band was imminent, so I seized this opportunity to capture this moment in time before it passed us all by. “The family of bands and friends that Adam Bartlett put together through his label is very special to me, and the decade that I spent touring around the world with Mutilation Rites, that allowed me to make all these personal connections to people in this scene was a very important part of my life. He played guitar and bass in Mutilation Rites, Cobalt, Disassociate, and Knight Terror and currently plays in Overdose, Moral Panic, and Total Invasion. He is currently working on several more documentaries, the subject matter ranging from the origins of personal computers and the internet, a skateboard gang, and the garment industry. A Wandering Path, The Story of Gilead Media is the first doc he has directed and produced himself. He went on to edit Something in the Water, a doc about Long Island Hardcore whose release date is still TBD. He also co-produced, edited, sound recorded, and was a camera operator on The Color of Noise, a doc that focused on Tom Hazelmyer, 90’s Noise Rock and Amphetamine Reptile. DIRECTOR / PRODUCER / EDITOR / DIRECTOR OF PHOTOGRAPHYĭimmitt’s first documentary work was in 2007, when he co-edited Until The Light Takes Us, a documentary about Norwegian Black Metal, that focused on Mayhem, Darkthrone, and Burzum among other controversial subject matter. 8/18/2023 0 Comments Klenow fragment ligationFor problematic ligations or if the DNA concentration is unknown, it may be necessary to vary ratios and run multiple ligations. A ratio above 6:1 will promote the insertion of multiple fragments, while dropping below 2:1 will reduce ligation efficiency. Single-insert ligations are optimal when targeting an insert:vector ratio between 2 and 6. It is recommended that the reaction buffer be discarded after one year of storage at -20☌ and replaced with fresh buffer to ensure maximum performance. One Weiss Unit is approximately equivalent to 22 Enzymatics cohesive end units. One Enzymatics T4 DNA Ligase cohesive end unit is equivalent to approximately 3 cohesive end units as measured with a Lambda-Hind III DNA fragment substrate in 1X T4 DNA Ligase reaction buffer. Based on the correlation between the no template control C t values and standard curve data, the detection limit of this assay is <10 copies genome/sample. The acceptance criterion for the test is the threshold cycle count (C t) produced by the average of 3 replicate no template control samples. Replicate 5 µL samples of enzyme solution were denatured and screened in a TaqMan qPCR assay for the presence of contaminating E.coli genomic DNA using oligonucleotide primers corresponding to the 16S rRNA locus. The acceptance criteria for this test requires that the aggregate mass of contaminant bands in the concentrated sample do not exceed the mass of the protein of interest band in the dilute sample, confirming greater than 99% purity of the concentrated sample.Ī 50 µL reaction containing 10,000 cpm of a radiolabeled single-stranded DNA substrate and 10 µL of enzyme solution incubated for 4 hours at 37☌ resulted in less than 1.0% release of TCA-soluble counts.Ī 50 µl reaction containing 5,000 cpm of a radiolabeled double-stranded DNA substrate and 10 µL of enzyme solution incubated for 4 hours at 37&C resulted in less than 1.0% release of TCA-soluble counts.Ī 50 µL reaction containing 0.5 µg of pENZuC DNA and 10 µL of enzyme solution incubated for 4 hours at 37☌ resulted in no visually discernible conversion to nicked circular DNA as determined by agarose gel electrophoresis. Following electrophoresis, the gel was stained and the samples compared to determine physical purity. SDS-Page (Physical Purity Assessment) and SpecificationsĢ.0 µL of concentrated enzyme solution was loaded on a denaturing 4-20% Tris-Glycine SDS-PAGE gel flanked by a broad-range MW marker and 2.0 µL of a 1:100 dilution of the same enzyme species. The observed average measurement of 3 replicate samples was converted to mg/mL using an extinction coefficient of 54,050 and molecular weight of 55,292 Daltons. Protein Concentration (OD 280) MeasurementĪ 2.0 µL sample of enzyme was analyzed at OD 280 using a Nanodrop ND-1000 spectrophotometer standardized using a 2.0 mg/ml BSA sample (Pierce Cat #23209) and blanked with product storage solution. Reactions are incubated for 30 minutes at 23☌, stopped, and analyzed on a 1% agarose gel stained with ethidium bromide. Dilutions of enzyme were made in 1X T4 DNA Ligase Reaction Buffer and added to 20 µL reactions containing double stranded DNA fragments and 1X T4 DNA Ligase Reaction Buffer. Unit activity was measured using a 2-fold serial dilution method. * For a detailed summary of assay conditions and data, refer to the Quality Controls Analysis section. PRODUCT SPECIFICATION* Storage Temperature coli strain carrying the cloned T4 DNA Ligase gene.ġ unit is defined as the amount of T4 DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X T4 DNA Ligase Buffer following a 30 minute incubation at 23☌. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).Ī recombinant E. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. If you want to have more OSS chains, you will have to fulfill all the requirements under the OSS branch. The key is to having enough SP when you want to unlock later chains. After a successful record, you are allowed to cast the OSS skill chain slightly off timing in the real battle. You can only record 1 OSS chain per weapon. It should be noted that you cannot access the menu in multi. For example, you can do ABA, but not AA.Īfter you implement or record save the chains in the OSS console, you have a bigger OSS activation gap where you can chain the skills.Īccess the menu through the console. The sword skills cannot be used back to back, however you can reuse the same skill within a chain. When you deal the OSS combo, you cancel out the CD between each skills, this means that you can use sword skills fast without any delay.Īs long as you have enough SP, you can connect the sword skills to the maximum OSS cap allowed. Then you will find the door for an event. The activation timing is shown when you glow in purple. Then use the teleporter and you will back in Ginriogia Stairway Remain F1 Just walk staight from the teleporter or go to the southwest exit. You can link any sword skills on the battle field while the timing is active. Aescenarion 6 years ago 1 Just started and not feeling straight sword at all. Pick a monster with the ability to cancel your skills, spam stun against those mobs to quickly satisfy the requirement. Sword Art Online: Hollow Realization Your favorite weapon to use. The Stun from the square key also counts as a sword skill. Note: In patch 1.05, the damage of OSS is buffed. In this guide, we will discuss some strategies and basic for the OSS system. The OSS System stands for Original Sword Skill in Hollow Fragment. 8/18/2023 0 Comments Jing chen emoryShe is an elected member of the Retina Society. Northwestern University Feinberg School of Medicine JING CHEN. Yan holds memberships in the American Academy of Ophthalmology, the American Society of Retina Specialists, the Association for Research in Vision and Ophthalmology, and the Georgia Society of Ophthalmology. Brumley Jr Professor and Chairman, Emory University School of Medicine. She serves as the vitreo-retinal surgery fellowship director. She directs the inherited retinal disease unit at Emory Eye Center with a special interest in retinal degenerative diseases. Jiong Yan, MD's clinical and research interests include medical and surgical management of age-related macular degeneration, retinal detachment, diabetic retinopathy, and macular disease. Despite Chapter 15 Directives' (2010) 27 Emory Bankruptcy Development Journal 117, 132 (citing P Wood. Jing Chen, PhD, currently the Janet Davison Rowley Professor of Medicine, has been named as the Janet D. Section of Vitreoretinal Surgery & Diseases (Retina) Jing Chen's research while affiliated with Emory University and other places Overview What is this page This page lists the scientific contributions of an author, who either does not have a. Commonwealth and US Perspectives Jingchen Xu. |
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